US 12,065,697 B2
B-family DNA polymerase variant and kit comprising the same
Cheng-Yao Chen, Hsinchu (TW); Yi-Wen Cheng, Hsinchu (TW); Yu Ting Hung, Hsinchu (TW); and Wen-Ting Chen, Hsinchu (TW)
Assigned to Cheng-Yao Chen, Hsinchu (TW)
Filed by Cheng-Yao Chen, Hsinchu (TW)
Filed on Jun. 28, 2022, as Appl. No. 17/852,008.
Claims priority of provisional application 63/249,813, filed on Sep. 29, 2021.
Prior Publication US 2023/0107606 A1, Apr. 6, 2023
Int. Cl. C12Q 1/6874 (2018.01); C12N 9/12 (2006.01)
CPC C12Q 1/6874 (2013.01) [C12N 9/1252 (2013.01); C12Y 207/07007 (2013.01)] 18 Claims
 
1. A B-family DNA polymerase variant modified from a wild-type B-family DNA polymerase having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 and 17, wherein the amino acid L at position 408 of SEQ ID NO: 2, 3, or 4 is substituted with F, I, M, Q, S, H or Y;
the amino acid L at position 409 of SEQ ID NO: 5 is substituted with F, I, M, Q, S, H or Y;
the amino acid L at position 411 of SEQ ID NO: 6 is substituted with F, I, M, Q, S, H or Y;
the amino acid M at position 417 of SEQ ID NO: 7 is substituted with F, I, M, Q, S, H or Y;
the amino acid L at position 485 of SEQ ID NO: 8 is substituted with F, I, M, Q, S, H or Y;
the amino acid L at position 606 of SEQ ID NO: 9 is substituted with F, I, M, Q, S, H or Y;
the amino acid L at position 612 of SEQ ID NO: 10 is substituted with F, I, M, Q, S, H or Y;
the amino acid M at position 426 of SEQ ID NO: 11 is substituted with F, I, M, Q, S, H or Y;
the amino acid L at position 518 of SEQ ID NO: 12 is substituted with F, I, M, Q, S, H or Y;
the amino acid L at position 425 of SEQ ID NO: 13 is substituted with F, I, M, Q, S, H or Y;
the amino acid L at position 423 of SEQ ID NO: 14 is substituted with F, I, M, Q, S, H or Y;
the amino acid F at position 415 of SEQ ID NO: 15 is substituted with F, I, M, Q, S, H or Y;
the amino acid F at position 412 of SEQ ID NO: 16 is substituted with F, I, M, Q, S, H or Y; or
the amino acid F at position 253 of SEQ ID NO: 17 is substituted with F, I, M, Q, S, H or Y.