CPC C12Q 1/6816 (2013.01) [C12Q 1/6844 (2013.01)] | 22 Claims |
1. A kit for determining the presence or absence of analyte polynucleotides in a sample, the kit comprising:
an internal control polynucleotide;
one or more amplification reagents to co-amplify a first analyte polynucleotide and the internal control polynucleotide in a nucleic acid amplification reaction to produce an internal control amplicon, and, if the sample contains the first analyte polynucleotide, a first analyte amplicon; and
a first hybridization probe having a first label, the first hybridization probe being capable of forming a first detectable hybrid with the first analyte amplicon, but incapable of forming a detectable hybrid with the internal control amplicon,
a second hybridization probe having a second label, the second hybridization probe being capable of forming a second detectable hybrid with the internal control amplicon, but incapable of forming a detectable hybrid with the first analyte amplicon,
a negative calibrator comprising the internal control polynucleotide, wherein the negative calibrator does not comprise the first analyte polynucleotide, and
one or more standards, each standard comprising the internal control polynucleotide and a known amount of the first analyte polynucleotide,
wherein the first and second labels comprise identical labels.
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