CPC C12Q 1/6806 (2013.01) [C12Q 1/6816 (2013.01); C40B 40/06 (2013.01)] | 17 Claims |
1. A method, comprising
obtaining a formalin fixed paraffin-embedded (FFPE) sample from a subject, the FFPE sample comprising protein-DNA complexes;
treating the FFPE sample with a proteinase to isolate nucleic acids such that the protein-DNA complexes are not destroyed, such that a first double-stranded segment and a second double-stranded segment are held together independent of a phosphodiester backbone, and such that the first double-stranded segment and the second double-stranded segment each have at least one exposed nucleic acid end; and
deriving genomic structural information by analyzing nucleic acids of the protein-DNA complexes in the FFPE sample,
wherein the treating the FFPE sample with the proteinase does not comprise overnight proteinase treatment.
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