US 12,065,681 B2
Method of producing ribulose-1,5-bisphosphate oxygenase protein fibers
Andries Don Sebastian, Kathleen, GA (US); John Paul Mua, Advance, NC (US); David Neil McClanahan, Winston-Salem, NC (US); Sammy Eni Eni, Winston-Salem, NC (US); and Thaddeus J. Jackson, Summerfield, NC (US)
Assigned to R.J. Reynolds Tobacco Company, Winston-Salem, NC (US)
Filed by R.J. REYNOLDS TOBACCO COMPANY, Winston-Salem, NC (US)
Filed on May 5, 2022, as Appl. No. 17/737,389.
Application 16/936,221 is a division of application No. 15/622,994, filed on Jun. 14, 2017, granted, now 10,745,682, issued on Aug. 18, 2020.
Application 17/737,389 is a continuation of application No. 16/936,221, filed on Jul. 22, 2020, granted, now 11,352,614.
Prior Publication US 2022/0259582 A1, Aug. 18, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/88 (2006.01); A23J 1/00 (2006.01); A23J 3/14 (2006.01); A23J 3/26 (2006.01); A23J 3/34 (2006.01); A24B 15/12 (2006.01); A24B 15/16 (2020.01); A24B 15/24 (2006.01)
CPC C12N 9/88 (2013.01) [A23J 1/007 (2013.01); A23J 3/14 (2013.01); A23J 3/26 (2013.01); A23J 3/34 (2013.01); A24B 15/12 (2013.01); A24B 15/16 (2013.01); A24B 15/24 (2013.01); A24B 15/241 (2013.01); C12Y 401/01039 (2013.01)] 8 Claims
 
1. A method of producing one or more ribulose-1,5-bisphosphate oxygenase protein fibers, the method comprising:
obtaining ribulose-1,5-bisphosphate oxygenase protein;
combining the ribulose-1,5-bisphosphate oxygenase protein with one or more plasticizers;
heating the ribulose-1,5-bisphosphate oxygenase combined with the one or more plasticizers to up to about 140 degrees C. and forming a mixture; and
passing the mixture through an orifice to produce the one or more ribulose-1,5-bisphosphate oxygenase protein fibers.