	US 12,065,678 B2
	Constructs, compositions and methods thereof having improved genome editing efficiency and specificity
Ryan T. Gill, Denver, CO (US); Tanya Warnecke, Boulder, CO (US); and Dominika Joanna Jedrzejczyk, Copenhagen (DK)
Assigned to Danmarks Tekniske Universitet, Lyngby (DK)
Filed by DANMARKS TEKNISKE UNIVERSITET, KGS Lyngby (DK)
Filed on May 1, 2023, as Appl. No. 18/142,013.
Application 18/142,013 is a continuation of application No. 17/780,002, previously published as PCT/US2020/061850, filed on Nov. 23, 2020.
Claims priority of provisional application 62/941,392, filed on Nov. 27, 2019.
Prior Publication US 2023/0340438 A1, Oct. 26, 2023
Int. Cl. C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01)

CPC C12N 9/22 (2013.01) [C12N 15/11 (2013.01); C12N 15/90 (2013.01); C12N 15/902 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)]

19 Claims

1. A nucleic acid-guided nuclease system comprising:

an engineered nucleic acid-guided nuclease, wherein the engineered nucleic acid-guided nuclease comprises a polypeptide sequence having at least 100% homology to a polypeptide consisting of SEQ ID NO: 94 (ABW8), 29 (ABW3), 81 (ABW7), 107 (ABW9), 3 (ABW1), 16 (ABW2), 42 (ABW4), 55 (ABW5), or 68 (ABW6), or a polynucleotide encoding the sequence; and

a guide nucleic acid compatible with the nucleic acid-guided nuclease and comprising a guide sequence complementary to a target sequence in a target polynucleotide, or a polynucleotide encoding the guide nucleic acid.