	US 12,065,677 B2
	Method for the fermentative production of guanidinoacetic acid
Frank Schneider, Halle (DE); and Frank Jankowitsch, Sassenberg (DE)
Assigned to Evonik Operations GmbH, Essen (DE)
Appl. No. 18/004,327
Filed by Evonik Operations GmbH, Essen (DE)
PCT Filed Jun. 28, 2021, PCT No. PCT/EP2021/067676 § 371(c)(1), (2) Date Jan. 5, 2023, PCT Pub. No. WO2022/008280, PCT Pub. Date Jan. 13, 2022.
Claims priority of application No. 20184949 (EP), filed on Jul. 9, 2020.
Prior Publication US 2023/0227795 A1, Jul. 20, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/10 (2006.01); C12P 13/04 (2006.01)

CPC C12N 9/1003 (2013.01) [C12N 9/1096 (2013.01); C12P 13/04 (2013.01); C12Y 201/04001 (2013.01); C12Y 206/01002 (2013.01); C12Y 206/01004 (2013.01); C12Y 206/01044 (2013.01)]

28 Claims

1. A microorganism, comprising:

at least one gene coding for a protein having the function of a L-arginine:glycine amidinotransferase, and

at least one protein having the function of a glyoxylate aminotransferase,

wherein the at least one protein having the function of the glyoxylate aminotransferase comprises an amino acid sequence which is at least 80% homologous to an amino acid sequence selected from the group of: SEQ ID NO:2, SEQ ID NO:5, and SEQ ID NO:8, and

wherein the protein having the function of the L-arginine; olycine amidinotransferase comprises an amino acid sequence which is at least 80% homologous to the amino acid sequence according to SEQ ID NO: 11.