US 12,065,673 B2
Method for obtaining human brown/beige adipocytes
Frederic Deschaseaux, Toulouse (FR); Fabien Guilloton, Toulouse (FR); Sandra Muller, Toulouse (FR); Luc Sensebe, Joue les Tours (FR); Louis Casteilla, Toulouse (FR); and Audrey Carriere-Pazat, Lacroix-Falgarde (FR)
Assigned to ETABLISSEMENT FRANCAIS DU SANG, La Plaine Saint Denis (FR); INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE, Paris (FR); UNIVERSITE TOULOUSE III—PAUL SABATIER, Toulouse (FR); and CENTRE NATIONAL DE LA RECHERCHE SECIENTIFIQUE, Paris (FR)
Appl. No. 15/743,062
Filed by ETABLISSEMENT FRANCAIS DU SANG, La Plaine Saint Denis (FR); INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE, Paris (FR); UNIVERSITE TOULOUSE III—PAUL SABATIER, Toulouse (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
PCT Filed Jul. 8, 2016, PCT No. PCT/EP2016/066361
§ 371(c)(1), (2) Date Jan. 9, 2018,
PCT Pub. No. WO2017/009263, PCT Pub. Date Jan. 19, 2017.
Claims priority of application No. 15306153 (EP), filed on Jul. 10, 2015.
Prior Publication US 2018/0216070 A1, Aug. 2, 2018
Int. Cl. C12N 5/077 (2010.01); A61K 35/35 (2015.01); C12N 5/0775 (2010.01); G01N 33/50 (2006.01)
CPC C12N 5/0653 (2013.01) [A61K 35/35 (2013.01); C12N 5/0663 (2013.01); C12N 5/0667 (2013.01); G01N 33/50 (2013.01); G01N 33/5044 (2013.01); C12N 2501/105 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/33 (2013.01); C12N 2501/999 (2013.01); C12N 2502/115 (2013.01); C12N 2506/13 (2013.01); C12N 2506/1346 (2013.01); C12N 2513/00 (2013.01)] 20 Claims
 
1. An in vitro method of producing brown/beige adipocytes comprising:
contacting cells selected from white adipose tissue cells and mesenchymal stem cells, with a differentiation medium comprising serum, a glucocorticoid and a mix of growth factors comprising a growth factor of the Vascular Endothelial Growth Factor (VEGF) family, a growth factor of the Epidermal Growth Factor (EGF) family, an insulin-like growth factor and a growth factor of the Fibroblast Growth Factor (FGF) family, until obtaining adipogenic progenitor cells,
contacting said adipogenic progenitor cells with an adipogenic agent until obtaining brown/beige adipocytes, and
optionally recovering said brown/beige adipocytes.