US 12,065,669 B2
Methods and compositions for modulating a genome
Stephen Hoyt Cleaver, Boston, MA (US); Barrett Ethan Steinberg, Somerville, MA (US); Jacob Rosenblum Rubens, Cambridge, MA (US); Robert James Citorik, Somerville, MA (US); William Edward Salomon, West Roxbury, MA (US); and Zi Jun Wang, Arlington, MA (US)
Assigned to Flagship Pioneering Innovations VI, LLC, Cambridge, MA (US)
Filed by Flagship Pioneering Innovations VI, LLC, Cambridge, MA (US)
Filed on Sep. 14, 2023, as Appl. No. 18/467,392.
Application 18/467,392 is a continuation of application No. 17/929,455, filed on Sep. 2, 2022.
Application 17/929,455 is a continuation of application No. PCT/US2021/020943, filed on Mar. 4, 2021.
Claims priority of provisional application 63/035,674, filed on Jun. 5, 2020.
Claims priority of provisional application 62/985,264, filed on Mar. 4, 2020.
Prior Publication US 2024/0076698 A1, Mar. 7, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/90 (2006.01); C12N 9/12 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01)
CPC C12N 15/907 (2013.01) [C12N 9/1276 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] 26 Claims
 
1. A system for modifying DNA, comprising:
a) a polypeptide or a nucleic acid encoding the polypeptide, wherein the polypeptide comprises (i) a reverse transcriptase domain and (ii) an endonuclease domain, and wherein the polypeptide comprises an amino acid sequence at least 95% identical to the sequence of SEQ ID NO: 1988; and
b) a template RNA comprising:
(i) a 5′ UTR sequence that binds the polypeptide,
(ii) a 3′ UTR sequence that binds the polypeptide, and
(ii) a heterologous object sequence.