CPC C12N 15/907 (2013.01) [C12N 9/22 (2013.01); C12N 15/102 (2013.01); C12N 15/67 (2013.01); C12N 15/85 (2013.01); C12N 2310/315 (2013.01); C12N 2310/321 (2013.01); C12N 2310/322 (2013.01); C12N 2310/335 (2013.01)] | 6 Claims |
1. A genome editing system having enhanced gene editing efficiency comprising:
a) a guide RNA that hybridizes with a target sequence of a DNA molecule in a eukaryotic cell that contains the DNA molecule, and
b) an mRNA encoding a CRISPR from Prevotella and Francisella 1 (“Cpf1”) protein;
wherein the guide RNA comprises five 2′fluoro (2′F) ribose at the 3′termini (cr3′5), and wherein the mRNA encoding the Cpf1 protein is fully substituted with pseudouridine (Ψ).
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