	US 12,065,659 B2
	Nucleus-encoded male sterility through mutation in cytochrome P450 oxidase
Dietrich Borchardt, Einbeck (DE); Olaf Czarnecki, Berlin (DE); and Wolfgang Mechelke, Einbeck (DE)
Assigned to KWS SAAT SE & Co. KGaA, Einbeck (DE)
Filed by KWS SAAT SE & Co. KGaA, Einbeck (DE)
Filed on Jun. 30, 2022, as Appl. No. 17/854,373.
Application 17/854,373 is a division of application No. 16/092,613, granted, now 11,473,103, previously published as PCT/EP2017/058815, filed on Apr. 12, 2017.
Claims priority of application No. 102016106656.7 (DE), filed on Apr. 12, 2016.
Prior Publication US 2022/0372583 A1, Nov. 24, 2022
Int. Cl. C12N 15/82 (2006.01); C12N 9/02 (2006.01); C12N 15/113 (2010.01); C12Q 1/6895 (2018.01)

CPC C12N 15/8289 (2013.01) [C12N 9/0071 (2013.01); C12N 15/1137 (2013.01); C12Q 1/6895 (2013.01); C12N 2310/122 (2013.01); C12N 2310/14 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)]

1 Claim

1. A method for identifying a Beta vulgaris plant displaying a recessive, nucleus-encoded male sterile phenotype, comprising detecting a mutation in a cytochrome P450 oxidase gene, or a marker coupled to the mutation, wherein the cytochrome P450 oxidase gene is a gene selected from the group consisting of:

(a) a nucleotide sequence comprising SEQ ID NO: 1 or 2;

(b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:3; and

(c) a nucleotide sequence which codes for an amino acid sequence which has an identity of at least 95% with the full length of SEQ ID NO:3,

wherein the mutation is selected from the group consisting of:

a deletion of one or more nucleotides between nucleotide positions 1560 and 2095 of SEQ ID NO:1; and

a point mutation of one or more nucleotides between nucleotide positions 1560 and 2095 of SEQ ID NO:1,

wherein the cytochrome P450 oxidase gene is located in a segment on chromosome 1 between marker loci sxn2151s01 and sle3305s02, and wherein the sxn2151s01 marker sequence of SEQ ID NO:24 and the sle3305s02 marker sequence of SEQ ID NO:26 indicate the presence of a gst locus resulting in male sterility;

identifying the Beta vulgaris plant having the recessive, nucleus-encoded male sterile phenotype or indirectly selecting a Beta vulgaris plant having a fertile male phenotype; and

breeding or producing a descendant recessive, nucleus-encoded, male sterile plant from the identified Beta vulgaris plant having the recessive, nucleus-encoded male sterile phenotype, wherein the nucleus-encoded male sterile phenotype is used for recurrent selection; or

breeding or producing a hybrid plant from the identified Beta vulgaris plant having the recessive, nucleus-encoded male sterile phenotype; or

breeding or producing a plant with restored fertility from the identified Beta vulgaris plant having the recessive, nucleus-encoded male sterile phenotype; or

crossbreeding the identified Beta vulgaris plant having the recessive, nucleus-encoded male sterile phenotype in a resistance breeding program; or

producing seeds from the identified Beta vulgaris plant having the recessive, nucleus-encoded male sterile phenotype.