US 12,065,657 B2
Overcoming self-incompatibility in diploid plants for breeding and production of hybrids
David S. Douches, Okemos, MI (US); Felix E. Enciso-Rodriguez, East Lansing, MI (US); C. Robin Buell, Perry, MI (US); Daniel Zarka, East Lansing, MI (US); Satya Swathi Nadakuduti, Haslett, MI (US); and Norma C. Manrique-Carpintero, East Lansing, MI (US)
Assigned to BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, East Lansing, MI (US)
Appl. No. 17/250,407
Filed by BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, East Lansing, MI (US)
PCT Filed Jul. 16, 2019, PCT No. PCT/US2019/041993
§ 371(c)(1), (2) Date Jan. 18, 2021,
PCT Pub. No. WO2020/018528, PCT Pub. Date Jan. 23, 2020.
Claims priority of provisional application 62/698,363, filed on Jul. 16, 2018.
Prior Publication US 2022/0025394 A1, Jan. 27, 2022
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8287 (2013.01) [C12N 15/8213 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method for reducing self-incompatibility in a diploid potato plant comprising: introducing a mutation into an endogenous S RNAse gene that decreases the expression and/or activity of the endogenous S RNAse gene and/or protein in said plant or plant part, plant organ or plant cell of said plant, wherein the endogenous S RNAse gene comprises a nucleotide sequence having at least 99% sequence identity to SEQ ID NO: 1 or at least 95% sequence identity to SEQ ID NO: 2 or 3.