	US 12,065,656 B2
	Insecticidal proteins from plants
Jennifer Kara Barry, Ames, IA (US); Kevin Hayes, Urbandale, IA (US); Lu Liu, Palo Alto, CA (US); Eric Schepers, Port Deposit, MD (US); and Nasser Yalpani, Kelowna (CA)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US); and CORTEVA AGRISCIENCE LLC, Indianapolis, IN (US)
Filed on Apr. 22, 2021, as Appl. No. 17/237,489.
Application 17/237,489 is a division of application No. 16/594,657, filed on Oct. 7, 2019, granted, now 11,015,208.
Application 16/594,657 is a division of application No. 15/116,715, granted, now 10,480,007, issued on Nov. 18, 2019, previously published as PCT/US2015/014816, filed on Feb. 6, 2015.
Claims priority of provisional application 61/937,288, filed on Feb. 7, 2014.
Prior Publication US 2021/0254095 A1, Aug. 19, 2021
Int. Cl. C12N 15/82 (2006.01); A01N 47/44 (2006.01); A01N 63/10 (2020.01); A01N 63/50 (2020.01); A01N 65/00 (2009.01); C07K 14/415 (2006.01); A01N 65/04 (2009.01)

CPC C12N 15/8286 (2013.01) [A01N 47/44 (2013.01); A01N 63/10 (2020.01); A01N 63/50 (2020.01); A01N 65/00 (2013.01); C07K 14/415 (2013.01); A01N 65/04 (2013.01); C07K 2319/00 (2013.01); Y02A 40/146 (2018.01)]

15 Claims

1. A recombinant Pteridophyta Insecticidal Protein-65 (PtIP-65) polypeptide operably linked to a heterologous signal or leader sequence, wherein said PtIP-65 polypeptide comprises an amino acid sequence having at least 95% sequence identity, across the entire length of the amino acid sequence, compared to the amino acid sequence of SEQ ID NO: 29, wherein said PtIP-65 polypeptide has insecticidal activity against an insect selected from the group consisting of Soy Bean Looper, Corn Earworm, European Corn Borer and Velvet Bean Caterpillar in combination with a PtIP-50 polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO: 84.