US 12,065,640 B2
Microfluidic device for extracting, isolating, and analyzing DNA from cells
Harold G. Craighead, Ithaca, NY (US); Juraj Topolancik, San Jose, CA (US); Harvey Tian, Fayetteville, AR (US); and Christopher Wallin, Ithaca, NY (US)
Assigned to CORNELL UNIVERSITY, Ithaca, NY (US)
Filed by CORNELL UNIVERSITY, Ithaca, NY (US)
Filed on Mar. 6, 2021, as Appl. No. 17/194,253.
Application 17/194,253 is a continuation of application No. 15/936,349, filed on Mar. 26, 2018, granted, now 10,947,528.
Application 15/936,349 is a continuation of application No. 14/124,479, granted, now 9,926,552, previously published as PCT/US2012/041136, filed on Jun. 6, 2012.
Claims priority of provisional application 61/548,896, filed on Oct. 19, 2011.
Claims priority of provisional application 61/493,748, filed on Jun. 6, 2011.
Prior Publication US 2021/0189381 A1, Jun. 24, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12Q 1/6806 (2018.01)
CPC C12N 15/1017 (2013.01) [B01L 3/502761 (2013.01); C12M 47/10 (2013.01); C12Q 1/6806 (2013.01); B01L 2200/0663 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/086 (2013.01); Y10T 29/49826 (2015.01)] 10 Claims
OG exemplary drawing
 
1. A system for analyzing DNA from a cell, the system comprising:
a support comprising an inlet port;
a microfluidic channel extending from the inlet port within the support;
a micropillar array in the microfluidic channel, the micropillar array comprising:
a first region of micropillars spatially configured to entrap a cell by size exclusion, and
a second region of micropillars spatially configured to immobilize DNA that is released upon lysis of the cell entrapped in the first region of micropillars due to the DNA looping around said micropillars of the second region when hydrodynamic force is applied; and
a fluorospectrometer or a fluorescent microscope operable to capture a fluorescent signal of the DNA released from the cell.