US 12,065,486 B2
VHH based binding antibodies for anthrax and botulinum toxins and methods of making and using therefor
Charles B. Shoemaker, Medford, MA (US)
Assigned to Trustees of Tufts College, Medford, MA (US)
Filed by TRUSTEES OF TUFTS COLLEGE, Medford, MA (US)
Filed on Apr. 2, 2021, as Appl. No. 17/221,177.
Application 17/221,177 is a division of application No. 15/534,776, granted, now 11,001,625, previously published as PCT/US2015/064872, filed on Dec. 10, 2015.
Claims priority of provisional application 62/089,949, filed on Dec. 10, 2014.
Prior Publication US 2021/0221874 A1, Jul. 22, 2021
Int. Cl. A61K 39/00 (2006.01); C07K 16/12 (2006.01)
CPC C07K 16/1282 (2013.01) [C07K 16/1278 (2013.01); C12Y 304/24069 (2013.01); C12Y 304/24083 (2013.01); A61K 2039/505 (2013.01); C07K 2317/31 (2013.01); C07K 2317/569 (2013.01); C07K 2317/76 (2013.01); G01N 2333/952 (2013.01); G01N 2333/954 (2013.01)] 11 Claims
 
1. A recombinant binding protein that specifically binds to a botulinum toxin B, wherein the binding protein comprises the amino acid sequence selected from the group consisting of SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, and a combination thereof.