US 12,065,467 B2
High-transduction-efficiency rAAV vectors, compositions, and methods of use
Arun Srivastava, Gainesville, FL (US); George Vladimirovich Aslanidi, Gainesville, FL (US); Kim M. Van Vliet, Gainesville, FL (US); and Mavis Agbandje-McKenna, Gainesville, FL (US)
Assigned to University of Florida Research Foundation, Incorporated, Gainesville, FL (US)
Filed by University of Florida Research Foundation, Incorporated, Gainesville, FL (US)
Filed on Jul. 5, 2022, as Appl. No. 17/810,729.
Application 16/283,705 is a division of application No. 15/444,235, filed on Feb. 27, 2017, granted, now 10,294,281.
Application 15/444,235 is a division of application No. 14/401,442, granted, now 9,611,302, previously published as PCT/US2013/041234, filed on May 15, 2013.
Application 17/810,729 is a continuation of application No. 16/283,705, filed on Feb. 22, 2019, granted, now 11,434,260.
Application 14/401,442 is a continuation in part of application No. 13/840,224, filed on Mar. 15, 2013, granted, now 9,725,485.
Claims priority of provisional application 61/647,318, filed on May 15, 2012.
Prior Publication US 2023/0129762 A1, Apr. 27, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 7/00 (2006.01); C07K 14/005 (2006.01); C12N 15/86 (2006.01)
CPC C07K 14/005 (2013.01) [C12N 15/86 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14145 (2013.01)] 21 Claims
OG exemplary drawing
 
1. An AAV VP3 protein comprising a non-native, non-threonine amino acid residue at a position corresponding to T491 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and:
(i) a non-native, non-serine amino acid residue at a position corresponding to S662 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(ii) a non-native, non-serine amino acid residue at a position corresponding to S662 and a non-native, non-tyrosine amino acid residue at a position corresponding to Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(iii) a non-native, non-tyrosine amino acid residue at a position corresponding to Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(iv) a non-native, non-tyrosine amino acid residue at a position corresponding to Y444 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(v) non-native, non-tyrosine amino acid residues at positions corresponding to Y444 and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(vi) non-native, non-tyrosine amino acid residues at positions corresponding to Y444, Y500, and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2; and/or
(vii) non-native, non-tyrosine amino acid residues at positions corresponding to Y272, Y444, Y500, and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
wherein the non-native, non-threonine amino acid residue is not alanine.