CPC C07K 14/005 (2013.01) [C12N 15/86 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14145 (2013.01)] | 21 Claims |
1. An AAV VP3 protein comprising a non-native, non-threonine amino acid residue at a position corresponding to T491 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2, and:
(i) a non-native, non-serine amino acid residue at a position corresponding to S662 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(ii) a non-native, non-serine amino acid residue at a position corresponding to S662 and a non-native, non-tyrosine amino acid residue at a position corresponding to Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(iii) a non-native, non-tyrosine amino acid residue at a position corresponding to Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(iv) a non-native, non-tyrosine amino acid residue at a position corresponding to Y444 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(v) non-native, non-tyrosine amino acid residues at positions corresponding to Y444 and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
(vi) non-native, non-tyrosine amino acid residues at positions corresponding to Y444, Y500, and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2; and/or
(vii) non-native, non-tyrosine amino acid residues at positions corresponding to Y272, Y444, Y500, and Y730 of the wild-type AAV2 capsid protein set forth in SEQ ID NO: 2;
wherein the non-native, non-threonine amino acid residue is not alanine.
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