US 12,064,765 B2
Method and device for determining the concentration of analytes in whole blood
Kalpesh Mehta, Singapore (SG); and Mohiudeen Azhar, Bangalore (IN)
Assigned to Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Appl. No. 17/264,487
Filed by Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
PCT Filed Jul. 31, 2019, PCT No. PCT/US2019/044294
§ 371(c)(1), (2) Date Jan. 29, 2021,
PCT Pub. No. WO2020/033192, PCT Pub. Date Feb. 13, 2020.
Claims priority of provisional application 62/715,019, filed on Aug. 6, 2018.
Prior Publication US 2021/0291183 A1, Sep. 23, 2021
Int. Cl. B01L 3/00 (2006.01); B03C 5/00 (2006.01); B03C 5/02 (2006.01); G01N 21/17 (2006.01); G01N 21/31 (2006.01); G01N 27/447 (2006.01); G01N 33/49 (2006.01)
CPC B01L 3/502753 (2013.01) [B01L 3/502761 (2013.01); B03C 5/005 (2013.01); B03C 5/024 (2013.01); G01N 21/1717 (2013.01); G01N 21/31 (2013.01); G01N 27/44721 (2013.01); G01N 27/44791 (2013.01); G01N 33/4925 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0822 (2013.01); B01L 2400/0424 (2013.01); G01N 2021/1721 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A system for determining a concentration of one or more analytes in whole blood, the system comprising:
a transparent channel configured to carry whole blood;
a light source configured to emit light on the transparent channel;
an actuation module associable with the transparent channel, wherein the actuation module comprises a pair of electrodes associated with opposite ends of the transparent channel, wherein the pair of electrodes are configured to generate a dielectrophoretic environment in the transparent channel and thereby generate a cell-free plasma layer from the whole blood in the center of the transparent channel when the pair of electrodes are subjected to an electric field; and
an optical module associable with the transparent channel, wherein the optical module is configured to determine, within the transparent channel, blood gas concentrations in unseparated whole blood and free hemoglobin concentration in the cell-free plasma layer; and
wherein the optical module is configured to determine blood gas concentrations in the unseparated whole blood in the transparent channel by capturing light transmitted from the transparent channel and computing a change in the intensity of the transmitted light, wherein the change in the intensity correlates to the concentration of the blood gas in the whole blood,
wherein the optical module is configured to determine free hemoglobin concentration in the cell-free plasma layer in the transparent channel by capturing light transmitted from the transparent channel and computing a change in an intensity of the transmitted light, wherein the change in the intensity correlates to free hemoglobin in the whole blood.