	US 12,064,531 B2
	Pre-loadable dried biological heart valve and preparation method thereof
Yunbing Wang, Chengdu (CN); Yang Lei, Chengdu (CN); Gaocan Li, Chengdu (CN); Li Yang, Chengdu (CN); Hou-Sen Lim, Singapore (SG); Dajun Kuang, Zhejiang (CN); and Jincheng Yu, Hangzhou (CN)
Assigned to VENUS MEDTECH (HANGZHOU), INC., Hangzhou (CN); and Sichuan University, Chengdu (CN)
Filed by VENUS MEDTECH (HANGZHOU), INC., Zhejiang (CN); and Sichuan University, Chengdu (CN)
Filed on May 6, 2021, as Appl. No. 17/313,877.
Application 17/313,877 is a continuation of application No. PCT/CN2019/105345, filed on Sep. 11, 2019.
Prior Publication US 2021/0260247 A1, Aug. 26, 2021
Int. Cl. A61L 27/50 (2006.01); A61F 2/02 (2006.01); A61F 2/24 (2006.01); A61L 27/36 (2006.01)

CPC A61L 27/3687 (2013.01) [A61L 27/3625 (2013.01); A61L 27/3641 (2013.01); A61L 2430/20 (2013.01)]

13 Claims

1. A method for preparing a pre-loadable dried biological heart valve, comprising:

Step A: soaking a fresh animal pericardium in an aqueous solution of soluble elastin or glycosaminoglycan, and then subjecting the pericardium to a first cross-linking reaction in a mixed solution of carbodiimide or N-hydroxysuccinimide to allow the soluble elastin or glycosaminoglycan to bind to the pericardium via a chemical bond; and

Step B: subjecting the pericardium after the first cross-linking to a second cross-linking reaction in an aqueous glutaraldehyde solution, and then drying the pericardium after the second cross-linking, to obtain the pre-loadable dried biological heart valve.