US 12,064,492 B2
Cells loaded with gold nanoparticles for use in the diagnosis and/or treatment of melanoma
Angela Zoppi, Florence (IT); Mario Del Rosso, Pistoia (IT); Anna Laurenzana, Tricarico (IT); Gabriella Fibbi, Florence (IT); Francesca Margheri, Empoli (IT); Anastasia Chilla', Florence (IT); and Giancarlo Margheri, Rome (IT)
Assigned to Universita degli Studi di Firenze, Florence (IT)
Appl. No. 16/096,505
Filed by Consiglio Nazionale delle Ricerche, Rome (IT); Plasma Diagnostics and Technologies S.r.l., Pisa (IT); and Universita degli Studi di Firenze, Florence (IT)
PCT Filed May 3, 2017, PCT No. PCT/IB2017/052567
§ 371(c)(1), (2) Date Oct. 25, 2018,
PCT Pub. No. WO2017/191572, PCT Pub. Date Nov. 9, 2017.
Claims priority of application No. 102016000045932 (IT), filed on May 4, 2016.
Prior Publication US 2019/0142980 A1, May 16, 2019
Int. Cl. A61K 9/00 (2006.01); A61K 33/242 (2019.01); A61K 35/28 (2015.01); A61K 41/00 (2020.01); A61K 47/69 (2017.01); A61K 49/00 (2006.01); A61K 51/12 (2006.01); A61P 35/00 (2006.01)
CPC A61K 51/1203 (2013.01) [A61K 33/242 (2019.01); A61K 35/28 (2013.01); A61K 41/0052 (2013.01); A61K 47/6901 (2017.08); A61K 49/0065 (2013.01); A61P 35/00 (2018.01)] 13 Claims
OG exemplary drawing
 
1. A cell comprising:
said cell loaded with a mixture of nanoparticles exhibiting a peak of absorbance at about 810 nm measured by UV-Vis spectroscopy and nanoparticles exhibiting a peak of absorbance at about 520 nm measured by UV-Vis spectroscopy, wherein said cell is an endothelial colony forming cell and said nanoparticles exhibiting a peak of absorbance at about 810 nm and nanoparticles exhibiting a peak of absorbance at about 520 nm are nanoparticles with a core of gold stabilized by a coating agent,
wherein said nanoparticles exhibiting a peak of absorbance at about 810 nm have a diameter smaller than 100 nm,
wherein said coating agent is chitosan, and
wherein said nanoparticles exhibiting a peak of absorbance at about 810 nm exhibits a thermotransductive effect at about 808 nm with a laser.