US 12,064,447 B1
Lyophyllum decastes polysaccharide, preparation method and application thereof
Qi Wang, Jilin (CN); Ling Su, Jilin (CN); Yan Zhang, Jilin (CN); Yu Li, Jilin (CN); Min Gao, Jilin (CN); Xueyu Shang, Jilin (CN); and Tianci Wang, Jilin (CN)
Assigned to Jilin Agricultural University, Jilin (CN)
Filed by Jilin Agricultural University, Jilin (CN)
Filed on Dec. 28, 2023, as Appl. No. 18/399,599.
Application 18/399,599 is a continuation of application No. PCT/CN2023/131160, filed on Nov. 13, 2023.
Claims priority of application No. 202310226009.3 (CN), filed on Mar. 10, 2023.
Int. Cl. A61K 31/715 (2006.01); A61P 1/00 (2006.01); A61P 29/00 (2006.01); A61P 39/06 (2006.01)
CPC A61K 31/715 (2013.01) [A61P 1/00 (2018.01); A61P 29/00 (2018.01); A61P 39/06 (2018.01)] 1 Claim
 
1. A preparation method of a Lyophyllum decastes polysaccharide, comprising following steps:
(1) adding an aqueous solution containing EDTA-2Na into a fruiting body of Lyophyllum decastes, performing ultrasonic treatment, and then performing water extraction treatment to obtain an extractive solution;
(2) concentrating the extractive solution, adding anhydrous ethanol for precipitation, followed by centrifuging to obtain precipitates, then obtaining a crude Lyophyllum decastes polysaccharide; and
(3) conducting removal of proteins and dialysis of the crude Lyophyllum decastes polysaccharide to obtain the Lyophyllum decastes polysaccharide;
wherein in step (1), a concentration of the EDTA-2Na in the aqueous solution containing EDTA-2Na is 1 wt %; a material-liquid ratio of the fruiting body of the Lyophyllum decastes to the aqueous solution containing EDTA-2Na is 1 g:22 mL; a duration of the ultrasonic treatment is 30 min; a power of the ultrasonic treatment is 100 W; and a temperature of the water extraction treatment is 98° C.