	US 12,063,902 B2
	Method of regenerating cannabis
Moshe Arie Flaishman, Herzliya (IL); Reut Cohen Peer, Kiryat-Ono (IL); Oded Cohen, Nir Zvi (IL); and Samuel Bocobza, Tel Aviv (IL)
Assigned to The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center), Rishon-LeZion (IL)
Filed by The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center), Rishon-LeZion (IL)
Filed on Jul. 11, 2022, as Appl. No. 17/861,459.
Application 17/861,459 is a continuation of application No. 16/972,647, abandoned, previously published as PCT/IL2019/050653, filed on Jun. 6, 2019.
Claims priority of provisional application 62/681,697, filed on Jun. 7, 2018.
Prior Publication US 2022/0386547 A1, Dec. 8, 2022
Int. Cl. A01H 4/00 (2006.01); A01G 22/00 (2018.01); A01H 6/28 (2018.01); A01G 31/00 (2018.01)

CPC A01H 4/005 (2013.01) [A01G 22/00 (2018.02); A01H 4/008 (2013.01); A01H 6/28 (2018.05); A01G 31/00 (2013.01)]

11 Claims

1. A method of in-vitro propagating cannabis plant, the method comprising:

(a) culturing a cannabis explant comprising a meristem on a culture medium; and subsequently

(b) culturing said cannabis explant in a liquid medium, wherein at least a portion of a time period in said liquid medium is with a cell wall disruption agent is selected from the group consisting of pectinase, cutinase and a combination thereof; and subsequently

(c) culturing said cannabis explant under conditions which consist of a solid medium without said cell wall disrupting agent; wherein steps (a)-(c) are performed in a consecutive manner and repeated at least once until emergence of leaves suitable for regeneration.