US 11,735,404 B2
Method, device and system for the treatment of biological cryogenic samples by plasma focused ion beams
Alex De Marco, Melbourne (AU); Sergey Gorelick, Cheltenham (AU); Chad Rue, Portland, OR (US); Joseph Christian, Beaverton, OR (US); Kenny Mani, Beaverton, OR (US); Steven Randolph, Portland, OR (US); and Matthias Langhorst, Hameln (DE)
Assigned to FEI Company, Hillsboro, OR (US)
Filed by FEI Company, Hillsboro, OR (US)
Filed on Sep. 10, 2020, as Appl. No. 17/17,580.
Application 17/017,580 is a continuation of application No. 16/225,213, filed on Dec. 19, 2018, abandoned.
Prior Publication US 2021/0066056 A1, Mar. 4, 2021
Int. Cl. H01J 37/32 (2006.01); H01J 49/00 (2006.01); G01N 1/32 (2006.01)
CPC H01J 49/0027 (2013.01) [G01N 1/32 (2013.01); H01J 37/32009 (2013.01); H01J 2237/0807 (2013.01); H01J 2237/31745 (2013.01); H01J 2237/31749 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method of analyzing a biological sample, comprising:
providing at least one biological sample in frozen form;
milling the sample using a plasma ion beam comprising at least an O+ plasma to isolate at least a target in the sample from the rest of the sample, wherein the sample is milled at a mass removal rate of at least 100 μm3/second using the plasma ion beam, and wherein the target is cryogenically preserved for sensitive protein analysis; and
analyzing the isolated target with the sensitive protein analysis, wherein the sensitive protein analysis includes proteomic analysis and/or next-generation sequencing.