US 11,733,241 B2
Marker, immunoassay method, immunoassay reagent, method for assaying analyte, analyte measurement kit, and lateral-flow chromatographic test strip
Yasufumi Matsumura, Tokyo (JP); Yasushi Enomoto, Tokyo (JP); and Ryuzo Shinta, Tokyo (JP)
Assigned to NIPPON STEEL Chemical & Material Co., Ltd., Tokyo (JP)
Filed by NIPPON STEEL Chemical & Material Co., Ltd., Tokyo (JP)
Filed on Apr. 5, 2020, as Appl. No. 16/840,449.
Application 16/840,449 is a division of application No. 15/323,102, granted, now 10,690,665, previously published as PCT/JP2015/068759, filed on Jun. 30, 2015.
Claims priority of application No. 2014-136356 (JP), filed on Jul. 1, 2014; and application No. 2014-136357 (JP), filed on Jul. 1, 2014.
Prior Publication US 2020/0240987 A1, Jul. 30, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/558 (2006.01); G01N 33/543 (2006.01); G01N 33/553 (2006.01)
CPC G01N 33/558 (2013.01) [G01N 33/54346 (2013.01); G01N 33/553 (2013.01)] 10 Claims
 
1. A marker, comprising a resin-metal composite with a structure formed by immobilizing metal particles on a resin particle, characterized by
having constitution (A):
(A) the average particle size of the resin-metal composite being in a range of 300 nm to 1000 nm, and the average particle size of the metal particles being in a range of 1 nm to 80 nm,
wherein the metal particles comprise: metal particles having a portion embedded in the resin particle and a portion exposed from the resin particle, and metal particles adsorbed on a surface of the resin particle, wherein an amount of the metal particles adsorbed on the surface of the resin particle is 20 wt % or less relative to the total metal particles,
the metal particles are gold particles, and
the resin particle is a particle comprising a nitrogenous polymer, the nitrogenous polymer being selected from the group consisting of poly-2-vinylpyridine, poly-3-vinylpyridine, poly-4-vinylpyridine, poly-2-(diisopropylamino)ethyl methacrylate, polypyrrole, polyamide, polyamic acid, and polyimide.