US 11,733,131 B2
Tissue slice selection method, and embedded block preparing cassette
Shinobu Masuda, Tokyo (JP); Ryohei Suzuki, Tokyo (JP); and Yuriko Kitano, Higashimurayama (JP)
Assigned to NIHON UNIVERSITY, Tokyo (JP); and NICHIREI BIOSCIENCES INC., Tokyo (JP)
Appl. No. 16/609,074
Filed by NIHON UNIVERSITY, Tokyo (JP); and NICHIREI BIOSCIENCES INC., Tokyo (JP)
PCT Filed Apr. 27, 2018, PCT No. PCT/JP2018/017336
§ 371(c)(1), (2) Date Oct. 28, 2019,
PCT Pub. No. WO2018/199335, PCT Pub. Date Nov. 1, 2018.
Claims priority of application No. 2017-090793 (JP), filed on Apr. 28, 2017.
Prior Publication US 2020/0149999 A1, May 14, 2020
Int. Cl. G01N 1/06 (2006.01); G01N 1/30 (2006.01); G01N 1/36 (2006.01); G01N 33/52 (2006.01)
CPC G01N 1/06 (2013.01) [G01N 1/30 (2013.01); G01N 1/36 (2013.01); G01N 33/52 (2013.01)] 6 Claims
 
1. A tissue section selection method for selecting a tissue section to be inspected by specifically detecting a marker, the method comprising:
(a) an embedded block-preparing step for preparing an embedded block by embedding both a biological tissue fragment and a standard substance in an embedding agent;
(b) an embedded tissue section-preparing step for preparing an embedded tissue section having a sheet shape by slicing the embedded block, the embedded tissue section having a tissue section and a standard substance section embedded in the embedding agent and appearing on the surface thereof; and
(c) a tissue section-selecting step for selecting the tissue section to be inspected by comparing a light signal from the standard substance section with a calibration curve as a reference, and selecting the tissue section to be inspected when the tissue section is determined to have a predetermined thickness, wherein
the standard substance comprises a thickness standard that serves as a reference for the thickness of the tissue section, and
the calibration curve represents a relationship between thickness of the tissue section and a color tone of the standard substance.