US 11,732,269 B2
Recombinant maize B chromosome sequence and uses thereof
Randall Kerstetter, Wildwood, MO (US); Jonathan C. Lamb, Wildwood, MO (US); Thomas S. Ream, Wildwood, MO (US); Shiaw-Pyng Yang, St. Louis, MO (US); and Xuefeng Zhou, St. Louis, MO (US)
Assigned to MONSANTO TECHNOLOGY LLC, St. Louis, MO (US)
Appl. No. 15/765,049
Filed by MONSANTO TECHNOLOGY LLC, St. Louis, MO (US)
PCT Filed Sep. 30, 2016, PCT No. PCT/US2016/054968
§ 371(c)(1), (2) Date Mar. 30, 2018,
PCT Pub. No. WO2017/059341, PCT Pub. Date Apr. 6, 2017.
Claims priority of provisional application 62/240,770, filed on Oct. 13, 2015.
Claims priority of provisional application 62/237,048, filed on Oct. 5, 2015.
Claims priority of provisional application 62/236,709, filed on Oct. 2, 2015.
Prior Publication US 2018/0273962 A1, Sep. 27, 2018
Int. Cl. C12N 15/82 (2006.01); C12Q 1/6895 (2018.01); C12N 15/64 (2006.01)
CPC C12N 15/8213 (2013.01) [C12N 15/64 (2013.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01)] 16 Claims
 
1. A recombinant maize B chromosome comprising at least one transgenic DNA of interest, wherein said at least one transgenic DNA of interest is adjacent to a left flank sequence on one side and a right flank sequence on the other side, and wherein at least one of said left and right flank sequences comprises a nucleic acid sequence having at least 90% sequence identity to at least 0.25 kb of SEQ ID NO: 881, and wherein the at least one transgenic DNA of interest encodes a peptide or an RNAi construct.