US 11,732,268 B2
Methods and compositions for use in genome modification in plants
Elysia K. Krieger, Kirkwood, MO (US); Richard J. Lawrence, Kirkwood, MO (US); Zarir E. Vaghchhipawala, Madison, WI (US); Jianping Xu, Chesterfield, MO (US); and Xudong Ye, Chesterfield, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Appl. No. 16/311,749
Filed by MONSANTO TECHNOLOGY LLC, St. Louis, MO (US)
PCT Filed Jun. 27, 2017, PCT No. PCT/US2017/039502
§ 371(c)(1), (2) Date Dec. 20, 2018,
PCT Pub. No. WO2018/005491, PCT Pub. Date Jan. 4, 2018.
Claims priority of provisional application 62/355,715, filed on Jun. 28, 2016.
Prior Publication US 2019/0211344 A1, Jul. 11, 2019
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8202 (2013.01) [C12N 15/8205 (2013.01); C12N 15/8213 (2013.01)] 16 Claims
 
1. A method of providing a sequence of interest to the genome of a plant cell, comprising contacting the plant cell with a Rhizobiales cell capable of transforming the plant cell,
wherein the Rhizobiales cell comprises at least one vector capable of forming two T-strands that are essentially complementary in at least a portion of the T-strands,
wherein the at least one vector comprises a first right border DNA sequence (RB1), a second right border DNA sequence (RB2), and at least one sequence of interest,
wherein the RB1 is positioned in the vector to initiate synthesis of a first T-strand such that the sequence of interest is in the sense orientation from the 5′ to 3′ end of the first T-strand; and the RB2 is positioned in the vector to initiate synthesis of a second T-strand such that the sequence of interest is in the anti-sense orientation relative to the sequence of interest in the first T-strand, and wherein the two T-strands resulting from initiation at RB1 and RB2 are essentially complementary in at least a portion of the sequence of interest, wherein the sequence of interest further comprises at least one homology arm DNA sequence, wherein the at least one homology arm DNA sequence comprises a sequence that is at least 80% identical to a target sequence in the plant genome and is at least 50 nucleotides in length, and
wherein the at least one vector does not comprise a left border DNA sequence.