CPC C12N 15/8202 (2013.01) [C12N 15/8205 (2013.01); C12N 15/8213 (2013.01)] | 16 Claims |
1. A method of providing a sequence of interest to the genome of a plant cell, comprising contacting the plant cell with a Rhizobiales cell capable of transforming the plant cell,
wherein the Rhizobiales cell comprises at least one vector capable of forming two T-strands that are essentially complementary in at least a portion of the T-strands,
wherein the at least one vector comprises a first right border DNA sequence (RB1), a second right border DNA sequence (RB2), and at least one sequence of interest,
wherein the RB1 is positioned in the vector to initiate synthesis of a first T-strand such that the sequence of interest is in the sense orientation from the 5′ to 3′ end of the first T-strand; and the RB2 is positioned in the vector to initiate synthesis of a second T-strand such that the sequence of interest is in the anti-sense orientation relative to the sequence of interest in the first T-strand, and wherein the two T-strands resulting from initiation at RB1 and RB2 are essentially complementary in at least a portion of the sequence of interest, wherein the sequence of interest further comprises at least one homology arm DNA sequence, wherein the at least one homology arm DNA sequence comprises a sequence that is at least 80% identical to a target sequence in the plant genome and is at least 50 nucleotides in length, and
wherein the at least one vector does not comprise a left border DNA sequence.
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