US 11,732,258 B2
Engineered guide RNA sequences for in situ detection and sequencing
Reza Kalhor, East Boston, MA (US); Prashant G. Mali, La Jolla, CA (US); and George M. Church, Brookline, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Appl. No. 16/346,569
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
PCT Filed Nov. 1, 2017, PCT No. PCT/US2017/059549
§ 371(c)(1), (2) Date May 1, 2019,
PCT Pub. No. WO2018/085414, PCT Pub. Date May 11, 2018.
Claims priority of provisional application 62/428,010, filed on Nov. 30, 2016.
Claims priority of provisional application 62/416,401, filed on Nov. 2, 2016.
Prior Publication US 2019/0264202 A1, Aug. 29, 2019
Int. Cl. C12N 15/00 (2006.01); C12N 15/11 (2006.01); C12N 15/10 (2006.01); C12Q 1/6853 (2018.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01)
CPC C12N 15/11 (2013.01) [C12N 9/22 (2013.01); C12N 15/10 (2013.01); C12N 15/1096 (2013.01); C12N 15/113 (2013.01); C12Q 1/6853 (2013.01); C12N 2310/20 (2017.05); C12N 2310/3519 (2013.01); C12N 2310/531 (2013.01)] 12 Claims
 
1. An engineered guide RNA sequence comprising a spacer sequence and a guide RNA scaffold sequence, wherein the guide RNA scaffold sequence comprises a reverse transcription primer docking site sequence complementary to a locus specific reverse-transcription primer.