US 11,732,251 B2
Anti-CRISPR polynucleotides and polypeptides and methods of use
Christophe Fremaux, Poitiers (FR); Philippe Horvath, Châtellerault (FR); Alexander Hynes, Québec City (CA); Marie-Laurence Lemay, Quebec City (CA); Sylvain Moineau, Quebec City (CA); Dennis A. Romero, Oregon, WI (US); Geneviève Rousseau, Québec City (CA); and Joshua K Young, Johnston, IA (US)
Assigned to DUPONT NUTRITION BIOSCIENCES APS, Kongens Lyngby (DK); and UNIVERSITE LAVAL (CANADA), Quebec (CA)
Appl. No. 16/607,849
Filed by DUPONT NUTRITION BIOSCIENCES APS, Copenhagen (DK); and UNIVERSITE LAVAL (CANADA), Québec (CA)
PCT Filed Apr. 24, 2018, PCT No. PCT/EP2018/060510
§ 371(c)(1), (2) Date Oct. 24, 2019,
PCT Pub. No. WO2018/197520, PCT Pub. Date Nov. 1, 2018.
Claims priority of provisional application 62/488,969, filed on Apr. 24, 2017.
Claims priority of provisional application 62/488,981, filed on Apr. 24, 2017.
Claims priority of provisional application 62/510,896, filed on May 25, 2017.
Claims priority of provisional application 62/510,914, filed on May 25, 2017.
Prior Publication US 2020/0190492 A1, Jun. 18, 2020
Int. Cl. C12N 9/22 (2006.01); C07K 14/005 (2006.01); C07K 14/315 (2006.01); C12N 15/63 (2006.01)
CPC C12N 9/22 (2013.01) [C07K 14/005 (2013.01); C07K 14/315 (2013.01); C12N 15/63 (2013.01); C12N 2795/10322 (2013.01)] 4 Claims
 
1. A method for modulating the activity of a Cas endonuclease with a target polynucleotide in a cell, comprising providing an anti-CRISPR (ACR) polypeptide having an amino acid sequence at least 90% identical to SEQ ID NO:8 to the cell, wherein the ACR polypeptide decreases the activity of the Cas endonuclease in the cell compared to a control cell comprising the Cas endonuclease but not comprising the ACR polypeptide.