US 11,732,227 B2
Cell culture vessels with stabilizer devices
William Joseph Lacey, North Andover, MA (US); Gregory Roger Martin, Acton, ME (US); and Allison Jean Tanner, Portsmouth, NH (US)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Appl. No. 16/629,656
Filed by CORNING INCORPORATED, Corning, NY (US)
PCT Filed Jul. 13, 2018, PCT No. PCT/US2018/041974
§ 371(c)(1), (2) Date Jan. 9, 2020,
PCT Pub. No. WO2020/013845, PCT Pub. Date Jan. 16, 2020.
Prior Publication US 2021/0230528 A1, Jul. 29, 2021
Int. Cl. C12M 1/24 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01)
CPC C12M 23/08 (2013.01) [C12M 23/34 (2013.01); C12M 25/00 (2013.01)] 29 Claims
OG exemplary drawing
 
1. A cell culture vessel, comprising:
a vessel body that defines a cell culture chamber enclosed between a bottom wall and a top wall;
a support column within the cell culture chamber extending between the top wall and the bottom wall;
a stabilizer device covering a width and length of the cell culture chamber having a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber;
wherein the support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation; and
wherein the vessel body includes a shelf that extends into the cell culture chamber and a cell culture surface positioned within the cell culture chamber, the shelf located above the cell culture chamber to engage the stabilizer device and inhibit movement of the stabilizer device toward the cell culture surface.
 
16. A cell culture vessel, comprising:
a vessel body that defines a cell culture chamber enclosed between a bottom wall and a top wall;
a support column within the cell culture chamber extending between the top wall and the bottom wall;
a stabilizer device covering a width and length of the cell culture chamber having a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber;
wherein the support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation; and
wherein the stabilizer device comprises a grid comprising a plurality of interconnected grid segments, a first stabilizer wall that extends between opposite ends of the grid and a second stabilizer wall that extends between opposite sides of the grid, wherein the first stabilizer wall is perpendicular to the second stabilizer wall.