CPC A61K 35/17 (2013.01) [A61K 39/0011 (2013.01); A61P 35/00 (2018.01); C07K 16/2803 (2013.01); C12N 5/0636 (2013.01); C12N 5/0638 (2013.01); A61K 2039/5158 (2013.01); C07K 2317/622 (2013.01); C07K 2319/03 (2013.01); C07K 2319/33 (2013.01); C12N 2510/00 (2013.01)] | 19 Claims |
1. A method of maintaining or increasing the proliferation rate of chimeric antigen receptor-expressing T-cells (CAR T-cell) and maintaining or increasing IL-2 level during CAR T-cell cancer therapy, the method comprising a step of administering to a human subject undergoing autologous or allogeneic CAR T-cell cancer therapy a composition comprising a preparation of human autologous or allogeneic mononuclear-enriched early apoptotic cells, or a supernatant of human autologous or allogeneic mononuclear-enriched early apoptotic cells, wherein said preparation of human autologous or allogeneic mononuclear-enriched early apoptotic cells is ≥40% AnnexinV+and ≤15% propidium iodide+, and wherein said human autologous or allogeneic mononuclear-enriched early apoptotic cells are peripheral blood mononuclear cells, wherein said proliferation rate of CAR T-cells and said IL-2 level are maintained or increased in the subject compared with a subject undergoing autologous or allogeneic CAR T-cell cancer therapy and not administered said preparation of human autologous or allogeneic mononuclear-enriched early apoptotic cells, or said supernatant of human autologous or allogeneic mononuclear-enriched early apoptotic cells.
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