US 12,394,223 B2
Methods and systems for computational decoding of biological, chemical, and physical entities
Jarrett D. Egertson, Rancho Palos Verdes, CA (US); Vadim Lobanov, Seattle, WA (US); David Stern, San Carlos, CA (US); Parag Mallick, San Mateo, CA (US); Sujal M. Patel, Seattle, WA (US); and Ryan K. Seghers, Kirkland, WA (US)
Assigned to Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed by Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed on Feb. 8, 2024, as Appl. No. 18/437,147.
Application 18/437,147 is a continuation of application No. 17/344,769, filed on Jun. 10, 2021, granted, now 11,935,311.
Claims priority of provisional application 63/037,747, filed on Jun. 11, 2020.
Prior Publication US 2024/0249539 A1, Jul. 25, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G06V 20/69 (2022.01); C12Q 1/6876 (2018.01); G01N 21/64 (2006.01); G06T 7/00 (2017.01); G06T 7/11 (2017.01); G06V 10/25 (2022.01); G06N 20/00 (2019.01)
CPC G06V 20/69 (2022.01) [C12Q 1/6876 (2013.01); G01N 21/6428 (2013.01); G06T 7/0012 (2013.01); G06T 7/11 (2017.01); G06V 10/25 (2022.01); G01N 2021/6439 (2013.01); G06N 20/00 (2019.01); G06T 2207/20081 (2013.01); G06T 2207/30072 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for detecting an array of proteins, comprising:
(a) using a set of light sensing devices, (i) acquiring a first set of pixel information from sites in an array, and (ii) acquiring a second set of pixel information from the sites in the array, wherein the sites comprise proteins, wherein the sites further comprise labels that produce light;
(b) processing the pixel information to (i) register the first set of pixel information and the second set of pixel information to a common coordinate system, and (ii) identify a set of regions of interest corresponding to the sites in the array that produce the light in the first set of pixel information and the second set of pixel information;
(c) classifying the pixel information for the set of regions of interest into a categorical classification from among a plurality of distinct categorical classifications, thereby producing a plurality of pixel classifications; and
(d) identifying a protein in the array of proteins based at least in part on the plurality of pixel classifications.