	US 12,392,787 B2
	Method for quantifying the amount of cholesterol in high-density lipoprotein 3
Maiko Higuchi, Gosen (JP); and Yasuki Itoh, Gosen (JP)
Assigned to DENKA COMPANY LIMITED, Tokyo (JP)
Filed by DENKA COMPANY LIMITED, Tokyo (JP)
Filed on Jan. 17, 2019, as Appl. No. 16/250,850.
Application 16/250,850 is a continuation of application No. 13/811,562, abandoned, previously published as PCT/JP2011/066672, filed on Jul. 22, 2011.
Claims priority of application No. 2010-166317 (JP), filed on Jul. 23, 2010.
Prior Publication US 2019/0154711 A1, May 23, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/92 (2006.01); C12Q 1/44 (2006.01); C12Q 1/60 (2006.01)

CPC G01N 33/92 (2013.01) [C12Q 1/44 (2013.01); C12Q 1/60 (2013.01); G01N 2333/918 (2013.01); G01N 2405/04 (2013.01); G01N 2405/08 (2013.01); G01N 2800/323 (2013.01)]

14 Claims

1. A method of quantifying cholesterol in high-density lipoprotein 3 (HDL3), said method comprising:

reacting a test sample with phospholipase and/or sphingomyelinase;

simultaneously or subsequently reacting the test sample with a first surfactant that reacts with lipoproteins other than HDL3 to eliminate or protect cholesterol and any esters thereof; and

subsequently reacting the test sample with a second surfactant that reacts with HDL3 to quantify remaining cholesterol in the test sample.