US 12,392,780 B2
Support for fluorescence polarization immunoassay, fluorescence polarization immunoassay kit and fluorescence polarization immunoassay
Ayuko Imai, Kanagawa (JP); Ken Sumiyoshi, Kanagawa (JP); and Masanori Shirokawa, Kanagawa (JP)
Assigned to TIANMA JAPAN, LTD., Kanagawa (JP)
Filed by TIANMA JAPAN, LTD., Kanagawa (JP)
Filed on Jan. 5, 2022, as Appl. No. 17/647,052.
Claims priority of application No. 2021-001841 (JP), filed on Jan. 8, 2021.
Prior Publication US 2022/0221465 A1, Jul. 14, 2022
Int. Cl. G01N 33/58 (2006.01); B01L 3/00 (2006.01); G01N 21/64 (2006.01); G01N 33/542 (2006.01)
CPC G01N 33/582 (2013.01) [B01L 3/5027 (2013.01); G01N 21/6428 (2013.01); G01N 33/542 (2013.01); B01L 2300/0829 (2013.01); G01N 2021/6439 (2013.01)] 19 Claims
 
1. A support for fluorescence polarization immunoassay of a target substance in a sample, the support comprising:
multiple recesses, the recesses being loaded with an antibody having a binding ability to the target substance and a fluorescent labeling substance in which the target substance is labeled with a fluorescent dye,
wherein the support comprises a plurality of types of the recesses resulting from difference in a loaded amount of the antibody and/or difference in a loaded amount of the fluorescent labeling substance,
wherein the support comprises multiple groups each including multiple homogeneous recesses, the multiple homogeneous recesses being loaded with a same substance and having a same loaded amount, and
wherein the antibody and the fluorescent labeling substance are bound to a surface of the recesses in a way that, when a sample solution containing the target substance is added to the recesses, the antibody and the fluorescent labeling substance can be released from the surface of the recesses into the sample solution.