US 12,392,770 B2
Foreign substance introduction construct and use thereof
Nahoko Baileykobayashi, Tsukuba (JP); and Tetsuhiko Yoshida, Tsukuba (JP)
Assigned to TOAGOSEI CO., LTD., Tokyo (JP)
Filed by TOAGOSEI CO., LTD., Tokyo (JP)
Filed on Mar. 3, 2023, as Appl. No. 18/177,931.
Claims priority of application No. 2022-035236 (JP), filed on Mar. 8, 2022.
Prior Publication US 2023/0280333 A1, Sep. 7, 2023
Int. Cl. G01N 33/50 (2006.01); C07K 7/08 (2006.01)
CPC G01N 33/5008 (2013.01) [C07K 7/08 (2013.01)] 9 Claims
 
1. A method for introducing a foreign substance of interest from outside a eukaryotic cell into at least a cytoplasm of the cell, the method comprising:
a step of preparing a foreign substance introduction construct that has
a carrier peptide fragment comprising either of the following amino acid sequences:
 
 (SEQ ID NO: 1)
 
 KKRTLRKSNRKKRWPC
 
 
 
 (SEQ ID NO: 2)
 
 KKRTLRKKKRKKRWPC
and
the foreign substance of interest bonded to an N-terminal side and/or C-terminal side of the carrier peptide fragment;
a step of supplying the foreign substance introduction construct into a sample that contains a target eukaryotic cell; and
a step of incubating the sample into which the foreign substance introduction construct has been supplied, to thereby introduce the construct into the eukaryotic cell in the sample.