US 12,392,694 B2
Apparatuses and methods for rapid collection of microbe from a sample
Javier Atencia, College Park, MD (US); Ethan Reggia, College Park, MD (US); and Drew Tack, College Park, MD (US)
Assigned to PathOtrak Inc., College Park, MD (US)
Appl. No. 17/603,248
Filed by PathOtrak, Inc., College Park, MD (US)
PCT Filed Apr. 12, 2020, PCT No. PCT/US2020/027863
§ 371(c)(1), (2) Date Oct. 12, 2021,
PCT Pub. No. WO2020/210772, PCT Pub. Date Oct. 15, 2020.
Claims priority of provisional application 62/932,168, filed on Nov. 7, 2019.
Claims priority of provisional application 62/833,317, filed on Apr. 12, 2019.
Prior Publication US 2022/0178799 A1, Jun. 9, 2022
Int. Cl. B01L 3/00 (2006.01); A61B 5/145 (2006.01); A61B 5/1477 (2006.01); B01F 33/302 (2022.01); B01F 33/3033 (2022.01); B01L 7/00 (2006.01); B33Y 80/00 (2015.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01); C12N 15/10 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); C12Q 1/689 (2018.01); G01N 1/40 (2006.01); G01N 21/29 (2006.01); G01N 21/77 (2006.01); G01N 21/78 (2006.01); G01N 21/84 (2006.01); G01N 33/493 (2006.01); G01N 33/52 (2006.01); G01N 33/543 (2006.01); G01N 35/00 (2006.01); G06T 7/00 (2017.01); G06T 7/73 (2017.01); G06T 7/90 (2017.01); G16H 10/40 (2018.01)
CPC G01N 1/4077 (2013.01) [C12N 15/1017 (2013.01); C12Q 1/689 (2013.01); G01N 2001/4088 (2013.01)] 26 Claims
OG exemplary drawing
 
1. An apparatus, comprising:
one or more sealable containers configured to receive a test sample comprising a liquid portion,
at least one outlet port, and
at least one filtration filter and at least one concentration filter fluidly connected in sequence, a filtrate from the at least one concentration filter being provided to the at least one outlet port to form a filtered outlet port,
wherein after the one or more sealable containers is sealed, the apparatus is configured to permit flow of the liquid portion of the test sample from the one or more sealable containers through the at least one filtered outlet port via the at least one filtration filter and the at least one concentration filter when a pressure difference of equal to or greater than about 4 pounds per square inch (psi) exists between the one or more sealable containers and the filtered outlet port,
wherein the test sample comprises or is suspected to comprise a microbe, and
wherein each of the at least one filtration filter and the at least one concentration filter has a pore size equal to or greater than the average size of the microbe.