US 12,392,085 B2
Use of in-situ ionic liquid (IL) and deep eutectic solvent (DES) synthesis using chemically synthesized or biomass-derived ions in the pretreatment of biomass
Harsha D. Magurudeniya, Newport News, VA (US); Ezinne Achinivu, Alexandria, VA (US); Blake A. Simmons, San Francisco, CA (US); and John M. Gladden, Alameda, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US); and NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC, Albuquerque, NM (US)
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US); and NATIONAL TECHNOLOGY AND ENGINEERING SOLUTIONS OF SANDIA, LLC, Albuquerque, NM (US)
Filed on Jun. 4, 2021, as Appl. No. 17/339,909.
Application 17/339,909 is a continuation in part of application No. 17/242,256, filed on Apr. 27, 2021.
Claims priority of provisional application 63/035,508, filed on Jun. 5, 2020.
Claims priority of provisional application 63/016,877, filed on Apr. 28, 2020.
Prior Publication US 2021/0363696 A1, Nov. 25, 2021
Int. Cl. D21C 3/20 (2006.01); C12P 19/02 (2006.01); C12P 19/14 (2006.01)
CPC D21C 3/20 (2013.01) [C12P 19/02 (2013.01); C12P 19/14 (2013.01); C12P 2201/00 (2013.01)] 17 Claims
 
1. A method to deconstruct a biomass: the method comprising:
(a) ensiling a biomass to produce an ensiled biomass comprising equal to or more than 10% by weight of one or more organic acids;
(b) introducing one or more components of an ionic liquid (IL) or deep eutectic solvent (DES) to the ensiled biomass, wherein the one or more components, and one or more organic acids already present in the ensiled biomass, form an ionic liquid (IL) or deep eutectic solvent (DES), or mixture thereof, which solubilizes the ensiled biomass to form a solubilized biomass mixture, and wherein the ensiled biomass has a solid loading of about 15% to about 40%, and the one or more components comprises an ethanolamine; and
(c) introducing an enzyme and/or a microbe to the solubilized biomass mixture such that the enzyme and/or microbe produces a sugar from the solubilized biomass mixture; wherein the ensiled biomass is an ensiled lignocellulosic biomass.