US 12,392,000 B2
Methods and compositions for producing brachytic corn plants
Ty Jason Barten, Ankeny, IA (US); Alana N. Brown, St. Louis, MO (US); Edward James Cargill, Chesterfield, MO (US); Romain Fouquet, Saint-Palais (FR); Jose Rafael Gomez, Zapopan (MX); Matthew Sean Marengo, Cary, NC (US); Manuel Oyervides Garcia, El Palomar (MX); Jeanette M. Peevers, Chesterfield, MO (US); and Dennis Hung Yang, Ballwin, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Nov. 30, 2021, as Appl. No. 17/538,145.
Application 17/538,145 is a division of application No. 16/589,702, filed on Oct. 1, 2019, granted, now 11,214,842.
Application 16/589,702 is a continuation of application No. 15/139,733, filed on Apr. 27, 2016, granted, now 10,472,684, issued on Nov. 12, 2019.
Claims priority of provisional application 62/180,430, filed on Jun. 16, 2015.
Claims priority of provisional application 62/153,831, filed on Apr. 28, 2015.
Prior Publication US 2022/0162713 A1, May 26, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6895 (2018.01); A01H 1/04 (2006.01); A01H 5/10 (2018.01); A01H 6/46 (2018.01)
CPC C12Q 1/6895 (2013.01) [A01H 1/045 (2021.01); A01H 5/10 (2013.01); A01H 6/4684 (2018.05); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/172 (2013.01)] 19 Claims
 
1. A method for selecting a corn plant or seed, said method comprising:
a. isolating a nucleic acid from a corn plant or seed;
b. analyzing said nucleic acid to detect a polymorphic marker associated with a brachytic haplotype, said brachytic haplotype comprising one or more brachytic alleles of markers, wherein said markers are selected from the group consisting of SEQ ID NOs: 5, 6, 8, 11 to 22, and 86 to 95;
c. selecting a corn plant or seed comprising said brachytic haplotype;
and wherein:
said brachytic allele at SEQ ID NO:5 comprises a 3 nucleotide deletion beginning at position 70,
said brachytic allele at SEQ ID NO:6 comprises a G nucleotide at position 70,
said brachytic allele at SEQ ID NO:8 comprises a GTCCGT nucleotide insertion beginning at position 72,
said brachytic allele at SEQ ID NO:11 comprises an A nucleotide at position 62,
said brachytic allele at SEQ ID NO:12 comprises a T nucleotide at position 70,
said brachytic allele at SEQ ID NO:13 comprises a T nucleotide at position 72,
said brachytic allele at SEQ ID NO:14 comprises a G nucleotide at position 72,
said brachytic allele at SEQ ID NO:15 comprises a G nucleotide at position 72,
said brachytic allele at SEQ ID NO:16 comprises an A nucleotide at position 72,
said brachytic allele at SEQ ID NO:17 comprises a C nucleotide at position 72,
said brachytic allele at SEQ ID NO:18 comprises a G nucleotide at position 72,
said brachytic allele at SEQ ID NO:19 comprises a C nucleotide at position 72,
said brachytic allele at SEQ ID NO:20 comprises a T nucleotide at position 72,
said brachytic allele at SEQ ID NO:21 comprises a C nucleotide at position 72,
said brachytic allele at SEQ ID NO:22 comprises a CTCG nucleotide insertion beginning at position 72,
said brachytic allele at SEQ ID NO:86 comprises a 6 nucleotide deletion beginning at position 72,
said brachytic allele at SEQ ID NO:87 comprises a TG nucleotide insertion beginning at position 76,
said brachytic allele at SEQ ID NO:88 comprises a GGTA nucleotide insertion beginning at position 66,
said brachytic allele at SEQ ID NO:89 comprises a 6 nucleotide deletion beginning at position 71,
said brachytic allele at SEQ ID NO:90 comprises a T nucleotide at position 77,
said brachytic allele at SEQ ID NO:91 comprises a TT nucleotide insertion beginning at position 60,
said brachytic allele at SEQ ID NO:92 comprises a multi-kilobase insertion beginning at position 63,
said brachytic allele at SEQ ID NO:93 comprises an 85 nucleotide deletion beginning at position 51,
said brachytic allele at SEQ ID NO:94 comprises an 18 nucleotide insertion beginning at position 52, or
said brachytic allele at SEQ ID NO:95 comprises a 579 nucleotide insertion beginning at position 66.