	US 12,391,999 B2
	Methods and kits for classifying cannabinoid production in cannabis plants
Kevin McKernan, Marblehead, MA (US)
Assigned to MEDICINAL GENOMICS CORPORATION, Beverly, MA (US)
Filed by MEDICINAL GENOMICS CORPORATION, Beverly, MA (US)
Filed on Jun. 14, 2021, as Appl. No. 17/346,709.
Application 17/346,709 is a division of application No. 16/221,171, filed on Dec. 14, 2018, granted, now 11,035,010.
Claims priority of provisional application 62/598,967, filed on Dec. 14, 2017.
Prior Publication US 2022/0017974 A1, Jan. 20, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6895 (2018.01); C12N 9/02 (2006.01)

CPC C12Q 1/6895 (2013.01) [C12N 9/0004 (2013.01); C12Y 121/03007 (2015.07); C12Y 121/03008 (2015.07); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01)]

20 Claims

1. A method of detecting whether a tetrahydrocannabinolic acid (THCA) synthase gene sequence is present in a Cannabis plant, comprising:

obtaining a sample collected from the Cannabis plant that contains nucleic acids;

contacting the nucleic acids with at least five primers specific for the THCA synthase gene sequence;

amplifying the THCA synthase gene sequence when present among the nucleic acids; and

detecting amplicons upon amplification of the THCA synthase gene sequence, wherein:

the primers specific for the THCA synthase gene sequence include a primer specific for a promoter sequence of the THCA synthase gene sequence;

amplification of the THCA synthase gene sequence indicates that the THCA synthase gene sequence is at least 2000 nucleotides long; and

a colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay is performed.