US 12,391,996 B2
Early warning genetic testing of toxic cyanobacteria in water supply
Jingrang Lu, Mason, OH (US)
Assigned to THE U.S. EPA, Washington, DC (US)
Filed by USA, represented by Administrator of the U.S. EPA, Washington, DC (US)
Filed on Sep. 26, 2018, as Appl. No. 16/142,319.
Prior Publication US 2024/0191311 A1, Jun. 13, 2024
Int. Cl. C12Q 1/689 (2018.01); A61K 35/748 (2015.01); C12Q 1/686 (2018.01)
CPC C12Q 1/689 (2013.01) [A61K 35/748 (2013.01); C12Q 1/686 (2013.01); C02F 2307/14 (2013.01); C12Q 2600/142 (2013.01)] 17 Claims
 
1. A qPCR and/or RT-qPCR cyanotoxin detection apparatus, comprising:
a first set of primers, comprising a first primer consisting of the sequence set forth in SEQ ID NO:1 and a second primer consisting of the sequence set forth in SEQ ID NO:2;
a second set of primers, comprising a third primer consisting of the sequence set forth in SEQ ID NO:21 and fourth primer consisting of the sequence set forth in SEQ ID NO:22;
a third set of primers, comprising a fifth primer consisting of the sequence set forth in SEQ ID NO: 23 and a sixth primer consisting of the sequence set forth in SEQ ID NO:24;
a fourth set of primers, comprising a seventh primer consisting of the sequence set forth in SEQ ID NO:27 and an eighth primer consisting of the sequence set forth in SEQID NO: 28, and
at least one probe comprising a detectable label.