US 12,391,994 B2
Reagent kit and markers for detecting renal cell carcinoma and method thereof
Junhong Wang, Hangzhou (CN); Qingxia Jiang, Hangzhou (CN); and Huijia Meng, Hangzhou (CN)
Assigned to Hangzhou York Biotech Co., LTD., Hangzhou (CN)
Filed by Hangzhou York Biotech Co., LTD., Hangzhou (CN)
Filed on Jun. 15, 2022, as Appl. No. 17/841,253.
Claims priority of application No. 202110674617.1 (CN), filed on Jun. 18, 2021.
Prior Publication US 2023/0092109 A1, Mar. 23, 2023
Int. Cl. C12Q 1/6886 (2018.01); C12Q 1/6851 (2018.01); G16H 50/20 (2018.01); G16H 50/30 (2018.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6886 (2013.01) [C12Q 1/6851 (2013.01); G16H 50/20 (2018.01); G16H 50/30 (2018.01); C12Q 1/6806 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/178 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A reagent kit for detecting renal cell carcinoma, comprising:
a set of primer-probe mixes for detecting miRNAs in exosomes, wherein:
the set of primer-probe mixes includes a reverse transcription primer R-23b for a specific reverse transcription miR-23b-5p target, a universal forward primer Ge—F for fluorescent PCR (polymerase chain reaction) detection, a specific reverse primer 23b-R, and a specific probe 23b-P; a reverse transcription primer R-34c for a specific reverse transcription miR-34c-5p target, a universal forward primer Ge—F for fluorescent PCR detection, a specific reverse primer 34c-R, and a specific probe 34c-P; a reverse transcription primer R-210 for a specific reverse transcription miR-210-3p target, a universal forward primer Ge—F for fluorescent PCR detection, a specific reverse primer 210-R, and a specific probe 210-P; and a reverse transcription primer R-508 for a specific reverse transcription miR-508-3p target, a universal forward primer Ge—F for fluorescent PCR detection, a specific reverse primer 508-R, and a specific probe 508-P; wherein the set of primer-probe mixes includes:
R-23b (SEQ ID NO: 1):
5′-GTGCTAAGCACAGCAGGGTCCGAGGTATTCGCTGTGCTTA
 
GCACGTGGTA-3′;
 
R-34c (SEQ ID NO: 2):
5′-CACGATTCGTGAGCAGGGTCCGAGGTATTCGCTCACGAAT
 
CGTGGCAATC-3′;
 
R-210 (SEQ ID NO: 3):
5′-TGCATCAGATGTGCAGGGTCCGAGGTATTCGCACATCTGA
 
TGCATCAGCC-3′;
 
R-508 (SEQ ID NO: 4):
5-CGTACAGTCCAGGCAGGGTCCGAGGTATTCGCCTGGACTGT
 
ACGTCTACTC-3′;
 
Ge-F (SEQ ID NO: 5):
5′-GCAGGGTCCGAGGTATTC-3′;
 
23b-R (SEQ ID NO: 6):
5′-CATCACATTGCCAGGGAT-3′;
 
34c-R (SEQ ID NO: 7):
5′-GCAGGCAGTGTAGTTAGCT-3′;
 
210-R (SEQ ID NO: 8):
5′-GGCTGTGCGTGTGACAGC-3′;
 
508-R (SEQ ID NO: 9):
5′-GCTGATTGTAGCCTTTTG-3′;
 
23b-P (SEQ ID NO: 10):
5′FAM-ACCACGTGCTAAGCACAG-MGB 3′;
 
34c-P (SEQ ID NO: 11):
5′VIC-GATTGCCACGATTCGTGAGC-MGB3′;
 
210-P (SEQ ID NO: 12):
5′ROX-GCTGATGCATCAGATGTG-MGB 3′;
and
 
508-P (SEQ ID NO: 13): 
5′CY5-AGTAGACGTACAGTCCAGG-MGB 3′.