	US 12,391,982 B2
	Optical discrimination apparatus and methods adapted to monitor reactions
Mohiudeen Azhar, Bangalore (IN); Paul Patt, Walnut Creek, CA (US); Ragavendar Madrassethuraman, Sharon, MA (US); and Karan Mohan, Union City, CA (US)
Assigned to Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Appl. No. 17/759,473
Filed by Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
PCT Filed Feb. 17, 2021, PCT No. PCT/US2021/070159 § 371(c)(1), (2) Date Jul. 26, 2022, PCT Pub. No. WO2021/189066, PCT Pub. Date Sep. 23, 2021.
Claims priority of provisional application 62/990,039, filed on Mar. 16, 2020.
Prior Publication US 2023/0064409 A1, Mar. 2, 2023
Int. Cl. C12Q 1/6844 (2018.01); G01N 21/47 (2006.01); G01N 21/64 (2006.01)

CPC C12Q 1/6844 (2013.01) [G01N 21/47 (2013.01); G01N 21/6428 (2013.01); C12Q 2600/16 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6439 (2013.01); G01N 2201/0634 (2013.01); G01N 2201/0635 (2013.01)]

11 Claims

1. An optical discrimination apparatus, comprising:

a multi-color light emitter configured to emit excitation light;

a sample holder, configured to hold extracted dye-marked nucleic acid fragments from a biological sample, located at a position configured to receive the excitation light emitted from the multi-color light emitter along a first direction;

light emission collection optics configured to collect scattered light and emission light from the sample holder along a second direction that is approximately orthogonal to the first direction;

a spectrally-dispersive element configured to spectrally disperse the scattered light and the emission light into dispersed light spectra; and

a spectral detector configured to receive at least some of the dispersed light spectra wherein the spectral detector is configured to receive at least some of the excitation light and at least some of the emission light on different photosites of the spectral detector.