US 12,391,971 B2
Method for synthesizing peptides in cell-free translation system
Shota Tanaka, Kanagawa (JP); Atsushi Ohta, Kanagawa (JP); and Tetsuo Kojima, Kanagawa (JP)
Assigned to CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo (JP)
Appl. No. 16/479,736
Filed by Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
PCT Filed Jan. 30, 2018, PCT No. PCT/JP2018/002831
§ 371(c)(1), (2) Date Jul. 22, 2019,
PCT Pub. No. WO2018/143145, PCT Pub. Date Aug. 9, 2018.
Claims priority of application No. 2017-015201 (JP), filed on Jan. 31, 2017.
Prior Publication US 2020/0040372 A1, Feb. 6, 2020
Int. Cl. C07K 1/06 (2006.01); C07H 21/02 (2006.01); C07K 17/00 (2006.01); C12P 21/02 (2006.01)
CPC C12P 21/02 (2013.01) [C07H 21/02 (2013.01); C07K 1/061 (2013.01); C07K 17/00 (2013.01)] 17 Claims
 
1. A method of synthesizing a peptide, wherein the method comprises a step of deprotecting a protecting group of an amino acid having the protecting group on the amino group that forms a peptide bond and a translation step, in a cell-free translation system that comprises tRNA to which the amino acid having the protecting group is linked, wherein the deprotecting and translation steps are performed in parallel, wherein the protecting group is

OG Complex Work Unit Chemistry
and wherein the protecting group is deprotected by a penicillin amidohydrolase.