	US 12,391,960 B2
	Methods and constructs for production of lentiviral vector
Young Shin, Pearland, TX (US); Anandita Seth, Pearland, TX (US); and Bingnan Gu, Pearland, TX (US)
Assigned to LONZA WALKERSVILLE, INC., Walkersville, MD (US)
Filed by LONZA WALKERSVILLE, INC., Walkersville, MD (US)
Filed on May 31, 2024, as Appl. No. 18/680,137.
Application 18/680,137 is a division of application No. 17/001,114, filed on Aug. 24, 2020, granted, now 12,037,599.
Claims priority of provisional application 62/890,904, filed on Aug. 23, 2019.
Prior Publication US 2024/0336936 A1, Oct. 10, 2024
Int. Cl. C12N 15/867 (2006.01); C07K 14/16 (2006.01); C12N 5/071 (2010.01); C12N 9/12 (2006.01); C12N 15/86 (2006.01)

CPC C12N 15/867 (2013.01) [C07K 14/161 (2013.01); C07K 14/162 (2013.01); C07K 14/163 (2013.01); C12N 5/0686 (2013.01); C12N 9/1241 (2013.01); C12N 15/8673 (2013.01); C12N 15/8676 (2013.01); C12N 15/86 (2013.01); C12N 2740/15051 (2013.01); C12N 2740/16052 (2013.01); C12N 2760/20222 (2013.01)]

13 Claims

1. A method of producing a lentiviral packaging vector-containing mammalian cell, comprising:

a. transfecting a mammalian cell with:

i. a packaging vector including an expression cassette, encoding:

1. a lentiviral regulator of expression of virion proteins (REV) gene under control of a first promoter;

2. a lentiviral envelope gene under control of a second promoter; and

3. a lentiviral group specific antigen (GAG) gene and a lentiviral polymerase (POL) gene both under control of a third promoter,

wherein the expression cassette is flanked on both the 5′ and 3′ ends by transposon-specific inverted terminal repeats (ITR); and

b. culturing the transfected mammalian cell; and

c. isolating the lentiviral packaging vector-containing mammalian cell.