US 12,391,957 B2
Recombinant Newcastle disease viruses and uses thereof for the prevention of RSV disease or human metapneumovirus disease
Joan E. Durbin, Sergeantsville, NJ (US); Russell K. Durbin, Sergeantsville, NJ (US); Adolfo Garcia-Sastre, New York, NY (US); Ignacio Mena, New York, NY (US); and Peter Palese, New York, NY (US)
Assigned to ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI, New York, NY (US); and RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY, New Brunswick, NJ (US)
Appl. No. 17/268,403
Filed by ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI, New York, NY (US); and RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY, New Brunswick, NJ (US)
PCT Filed Aug. 16, 2019, PCT No. PCT/US2019/046837
§ 371(c)(1), (2) Date Feb. 12, 2021,
PCT Pub. No. WO2020/037215, PCT Pub. Date Feb. 20, 2020.
Claims priority of provisional application 62/765,242, filed on Aug. 17, 2018.
Prior Publication US 2021/0198323 A1, Jul. 1, 2021
Int. Cl. C07K 14/08 (2006.01); A61K 39/12 (2006.01); C07K 16/10 (2006.01); C12N 15/113 (2010.01); C12N 15/86 (2006.01); A61K 39/00 (2006.01)
CPC C12N 15/86 (2013.01) [A61K 39/12 (2013.01); C07K 14/08 (2013.01); C12N 15/113 (2013.01); A61K 2039/505 (2013.01); A61K 2039/543 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A recombinant Newcastle disease virus (NDV) comprising a packaged genome comprising
(a) a transgene encoding a fusion (F) protein of a human virus of the family Pneumoviridae, wherein the transgene comprises
(i) a ribonucleic acid (RNA) sequence corresponding to the negative sense of the complementary deoxyribonucleic acid (cDNA) sequence of SEQ ID NO: 2, 26, 28, or 30, or
(ii) an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO: 18, 53, 55, or 57;
(b) a transgene encoding a chimeric protein comprising an ectodomain of a F protein of a human virus of the family Pneumoviridae and a transmembrane domain and a cytoplasmic domain of a NDV F protein, wherein:
(i) the human virus of the family Pneumoviridae is human respiratory syncytial virus (“RSV”), and the chimeric protein comprises the ectodomain of the amino acid sequence set forth in SEQ ID NO:7,
(ii) the transgene comprises an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO: 19, 32, 34, 35, or 36, or
(iii) the transgene comprises an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO: 4, 44, 45, or 46; or
(c) a transgene encoding a bovine respiratory syncytial virus (“RSV”) F protein, wherein the transgene comprises an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO: 11, 41, or 43; or
(d) a transgene encoding a chimeric protein comprising a bovine Respiratory Syncytial Virus (RSV) F protein ectodomain and a transmembrane domain and a cytoplasmic domain of a NDV F protein, wherein:
(i) the chimeric protein comprises the amino acid sequence set forth in SEQ ID NO:33, or
(ii) the transgene comprises an RNA sequence corresponding to the negative sense of the cDNA sequence of SEQ ID NO: 14, 31, 38, or 39.