US 12,391,949 B2
High-throughput cargo delivery into live cells using photothermal platforms
Yi-Chien Wu, Taichung (TW); Ting-Hsiang S. Wu, Culver City, CA (US); Pei-Yu E. Chiou, Los Angeles, CA (US); and Michael A. Teitell, Tarzana, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Mar. 16, 2021, as Appl. No. 17/203,620.
Application 17/203,620 is a division of application No. 14/771,478, granted, now 10,982,217, previously published as PCT/US2014/026618, filed on Mar. 13, 2014.
Claims priority of provisional application 61/799,222, filed on Mar. 15, 2013.
Prior Publication US 2021/0207150 A1, Jul. 8, 2021
Int. Cl. C12N 15/64 (2006.01); C12M 1/42 (2006.01); C12N 5/071 (2010.01); C12N 13/00 (2006.01); C12N 15/87 (2006.01)
CPC C12N 15/64 (2013.01) [C12M 35/02 (2013.01); C12N 5/0602 (2013.01); C12N 13/00 (2013.01); C12N 15/87 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A device for delivering an agent into a cell, the device comprising:
a rigid substrate comprising a first planar surface and a second planar surface on a side opposite the first planar surface and an array of micro-orifices, where the micro-orifices penetrate through the substrate from the first planar surface to the second planar surface, where the micro-orifices have a maximum diameter of less than about 10 μm and define a sidewall extending from the first planar surface to the second planar surface for each micro-orifice and at least a portion of the sidewall of the array of micro-orifices is coated with a thin film coating of a semiconductor or metal that heats up when exposed to optical radiation; and
a fluid channel or fluid reservoir disposed adjacent to the second planar surface where the channel or reservoir is in fluid communication with the array of micro-orifices; and
wherein the face of the first planar surface is disposed to receive and support and/or contain cells.