US 12,391,939 B2
Genetically-encoded bicyclic peptide libraries
Ratmir Derda, Edmonton (CA); Raja Mukherjee, Edmonton (CA); and Vivian Triana, Edmonton (CA)
Appl. No. 17/261,390
Filed by THE GOVERNORS OF THE UNIVERSITY OF ALBERTA, Edmonton (CA)
PCT Filed Jul. 23, 2019, PCT No. PCT/CA2019/051017
§ 371(c)(1), (2) Date Jan. 19, 2021,
PCT Pub. No. WO2020/019072, PCT Pub. Date Jan. 30, 2020.
Claims priority of provisional application 62/702,284, filed on Jul. 23, 2018.
Prior Publication US 2021/0340525 A1, Nov. 4, 2021
Int. Cl. C40B 40/10 (2006.01); C12N 15/10 (2006.01)
CPC C12N 15/1037 (2013.01) [C40B 40/10 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A genetically-encoded peptide construct comprising a bicyclic structure comprising a two-fold symmetric linker having a first end and a second end comprising two reactive groups attached to a genetically encoded polypeptide, wherein the first end is ligated to a terminus of the polypeptide by a covalent bond, and wherein both reactive groups of the linker second end are each ligated to side chain residues of the polypeptide by a covalent bond, wherein the linker comprises an aliphatic chain having between 1-6 carbon atoms, wherein the polypeptide consists of unprotected, natural proteogenic amino acids, and does not comprise a free N-terminus.