	US 12,391,937 B2
	Biosynthesis of products from 1-carbon compounds
Ramon Gonzalez, Tampa, FL (US); Alexander Chou, Houston, TX (US); and James Clomburg, Houston, TX (US)
Assigned to Mojia Biotech Pte. Ltd., Singapore (SG)
Filed by Mojia Biotech Pte. Ltd., Singapore (SG)
Filed on Oct. 26, 2021, as Appl. No. 17/510,641.
Application 17/510,641 is a continuation of application No. 15/523,284, granted, now 11,186,834, previously published as PCT/US2015/058121, filed on Oct. 29, 2015.
Claims priority of provisional application 62/069,850, filed on Oct. 29, 2014.
Prior Publication US 2022/0112477 A1, Apr. 14, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/88 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01); C12P 1/04 (2006.01); C12P 5/02 (2006.01); C12P 7/04 (2006.01); C12P 7/20 (2006.01); C12P 7/24 (2006.01); C12P 7/40 (2006.01); C12P 11/00 (2006.01); C12P 13/00 (2006.01)

CPC C12N 9/88 (2013.01) [C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12P 1/04 (2013.01); C12P 5/02 (2013.01); C12P 7/04 (2013.01); C12P 7/20 (2013.01); C12P 7/24 (2013.01); C12P 7/40 (2013.01); C12P 11/00 (2013.01); C12P 13/001 (2013.01); C12Y 401/00 (2013.01); Y02P 20/141 (2015.11)]

36 Claims

1. A method for the production of a product from a single carbon substrate, the method comprising the steps of:

a) growing a genetically modified microorganism in a culture comprising the single carbon substrate and a growth media,

b) converting said single carbon substrate to a formyl-CoA,

c) condensing an initial C(n)-aldehyde with the formyl-CoA to produce 2-hydroxy-C(n+1)-acyl-CoA, wherein the 2-hydroxy-C(n+1)-acyl-CoA is one carbon longer than the initial C(n) aldehyde and wherein n is the number of carbons in the initial C(n)-aldehyde;

d) converting the 2-hydroxy-C(n+1)-acyl-CoA to a C(n+1)-aldehyde;

e) isolating a product from said microorganism, or said growth media, or both;

wherein the single carbon substrate is selected from the group consisting of methane, methanol, formaldehyde, formate, carbon monoxide, carbon dioxide, and combinations thereof; and

wherein the microorganism expresses:

i. a 2-hydroxyacyl-CoA lyase or an oxalyl-CoA decarboxylase that catalyzes the condensation of the initial C(n)-aldehyde with the formyl-CoA to produce the 2-hydroxy-C(n+1)-acyl-CoA;

ii. one or more polypeptides that catalyze the conversion of the single carbon substrate to the formyl-CoA; and

iii. one or more polypeptides that catalyze a conversion of the 2-hydroxy-C(n+1)-acyl-CoA to the C(n+1)-aldehyde.