US 12,391,935 B2
Compositions comprising digestive enzymes
Halle Redfearn, Daly City, CA (US); Harshal Kshirsagar, Daly City, CA (US); Kritika Mahadevan, Daly City, CA (US); Alexandre Chapeaux, Daly City, CA (US); Wesley Rutherford-Jenkins, Daly City, CA (US); Joel Andrew Kreps, Daly City, CA (US); and Isha Joshi, Daly City, CA (US)
Assigned to CLARA FOODS CO., Daly City, CA (US)
Filed by Clara Foods Co., Daly City, CA (US)
Filed on Mar. 28, 2023, as Appl. No. 18/191,395.
Application 18/191,395 is a division of application No. 17/467,601, filed on Sep. 7, 2021, granted, now 11,649,445.
Application 17/467,601 is a continuation of application No. 17/190,173, filed on Mar. 2, 2021, granted, now 11,142,754, issued on Oct. 12, 2021.
Application 17/190,173 is a continuation of application No. PCT/US2020/045519, filed on Aug. 7, 2020.
Claims priority of provisional application 62/941,627, filed on Nov. 27, 2019.
Claims priority of provisional application 62/883,800, filed on Aug. 7, 2019.
Prior Publication US 2023/0332125 A1, Oct. 19, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/50 (2006.01); C12N 9/64 (2006.01); C12P 21/00 (2006.01)
CPC C12N 9/50 (2013.01) [C12N 9/6481 (2013.01); C12P 21/00 (2013.01); C12Y 304/23001 (2013.01)] 12 Claims
 
1. A method for producing a consumable composition comprising combining a high-activity stable pepsin powdered composition with one or more consumable ingredients comprising steps of:
(a) providing a microorganism expressing a recombinant pepsinogen, wherein the expressed pepsinogen is secreted by the microorganism into its growth media;
(b) harvesting the growth media and removing the microorganismal cells therefrom to obtain a liquid starting material;
(c) lowering the pH of the liquid starting material to less than pH 4.0 to obtain an activated pepsin composition;
(d) raising the activated pepsin composition to a pH of 5.4 or higher to obtain a high-activity stable pepsin composition; and
(e) isolating the stable pepsin polypeptide from protein and small molecules in the liquid starting material to obtain the high-activity stable pepsin powdered composition having a specific activity from 500 FCC units/mg total protein to 50,000 FCC units/mg total protein;
(f) combining the high-activity stable pepsin powdered composition with the one or more consumable ingredients to produce a consumable composition; and
wherein step (e) is performed after steps (c) and (d).