US 12,391,931 B2
Esterases and methods of using the same
Vincent Tournier, Toulouse (FR)
Assigned to CARBIOS
Appl. No. 17/625,782
Filed by CARBIOS, Clermont-Ferrand (FR)
PCT Filed Jul. 10, 2020, PCT No. PCT/EP2020/069506
§ 371(c)(1), (2) Date Jan. 10, 2022,
PCT Pub. No. WO2021/005199, PCT Pub. Date Jan. 14, 2021.
Claims priority of application No. 19185796 (EP), filed on Jul. 11, 2019.
Prior Publication US 2022/0282232 A1, Sep. 8, 2022
Int. Cl. C12N 9/16 (2006.01); C08J 11/10 (2006.01); C11D 3/386 (2006.01)
CPC C12N 9/16 (2013.01) [C08J 11/105 (2013.01); C11D 3/386 (2013.01)] 15 Claims
 
1. An esterase, (i) having at least 90% identity compared to the esterase having the full length amino acid sequence set forth in SEQ ID NO: 1, (ii) comprising at least the combination of substitutions N207C+S256C, (iii) having an increased degrading activity and/or an increased thermostability as compared to the esterase of SEQ ID NO: 1, and (iv) comprising the combination of amino acid residues S134+D180+H211 and the combination of amino acid residues C247+C263+C177+C213 as in the esterase having the full length amino acid sequence set forth in SEQ ID NO: 1.