US 12,391,930 B2
Polymerase compositions and kits, and methods of using and making the same
Daniel Mazur, San Diego, CA (US); Sihong Chen, Vista, CA (US); Peter Vander Horn, Encinitas, CA (US); Eileen Tozer, San Diego, CA (US); Guobin Luo, Carlsbad, CA (US); Joshua Shirley, Carlsbad, CA (US); and Kevin Heinemann, Carlsbad, CA (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Nov. 28, 2023, as Appl. No. 18/521,466.
Application 18/521,466 is a continuation of application No. 17/302,621, filed on May 7, 2021, granted, now 11,866,740.
Application 17/302,621 is a continuation of application No. 16/415,063, filed on May 17, 2019, granted, now 11,001,814, issued on May 11, 2021.
Application 16/415,063 is a continuation of application No. 15/277,834, filed on Sep. 27, 2016, granted, now 10,344,268, issued on Jul. 9, 2019.
Claims priority of provisional application 62/235,616, filed on Oct. 1, 2015.
Prior Publication US 2024/0101980 A1, Mar. 28, 2024
Int. Cl. C12N 9/12 (2006.01); C12Q 1/686 (2018.01)
CPC C12N 9/1252 (2013.01) [C12Q 1/686 (2013.01); C12Y 207/07007 (2013.01)] 17 Claims
 
1. A composition comprising a recombinant DNA polymerase, wherein the recombinant DNA polymerase comprises a polypeptide segment exhibiting polymerase activity, wherein said polypeptide segment has at least 99% identity to SEQ ID NO: 35, and said polypeptide segment has a single amino acid substitution selected from the group consisting of E220K, N234K, N234R, V241K, D264K, E294K, E325R, and N485R, wherein the numbering of said amino acid substitutions in the polypeptide segment is relative to SEQ ID NO: 35 and wherein the polypeptide segment has the amino acid sequence selected from the group consisting of SEQ ID NOs: 79, 81, 82, 86, 94, 101, 111, and 113.