US 12,391,918 B2
Media for culturing stem cells
Michal Amit, Yuvalim (IL); and Joseph Itskovitz-Eldor, Haifa (IL)
Assigned to Technion Research & Development Foundation Limited, Haifa (IL)
Filed by Technion Research & Development Foundation Limited, Haifa (IL)
Filed on Oct. 24, 2022, as Appl. No. 17/971,677.
Application 17/971,677 is a division of application No. 16/454,168, filed on Jun. 27, 2019, granted, now 11,512,283.
Application 16/454,168 is a division of application No. 15/209,776, filed on Jul. 14, 2016, granted, now 10,385,312, issued on Aug. 20, 2019.
Application 13/909,128 is a division of application No. 11/991,077, granted, now 8,476,070, issued on Jul. 2, 2013, previously published as PCT/IL2006/000998, filed on Aug. 29, 2006.
Application 15/209,776 is a continuation of application No. 13/909,128, filed on Jun. 4, 2013, granted, now 9,404,079, issued on Aug. 2, 2016.
Claims priority of provisional application 60/834,795, filed on Aug. 2, 2006.
Claims priority of provisional application 60/711,668, filed on Aug. 29, 2005.
Prior Publication US 2023/0060616 A1, Mar. 2, 2023
Int. Cl. C12N 5/074 (2010.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); C12N 5/073 (2010.01); C12N 5/0735 (2010.01)
CPC C12N 5/0606 (2013.01) [C12N 5/0018 (2013.01); C12N 5/0037 (2013.01); C12N 5/0043 (2013.01); C12N 5/0056 (2013.01); C12N 5/0602 (2013.01); C12N 5/0603 (2013.01); C12N 2500/90 (2013.01); C12N 2500/98 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01); C12N 2501/23 (2013.01); C12N 2501/2306 (2013.01); C12N 2506/02 (2013.01); C12N 2533/52 (2013.01)] 17 Claims
 
1. A method of generating lineage-specific cells from primate embryonic stem cells, the method comprising:
(a) culturing the primate embryonic stem cells in a protein carrier-free culture medium comprising basic fibroblast growth factor (bFGF) and at least one factor selected from the group consisting of: transforming growth factor beta-3 (TGFβ3) isoform, transforming growth factor beta-1 (TGFβ1) isoform and IL6RIL6 chimera, wherein said IL6RIL6 chimera comprises amino acids 112-355 of SEQ ID NO: 32,
to thereby obtain expanded, undifferentiated embryonic stem cells;
(b) subjecting said expanded, undifferentiated primate embryonic stem cells to culturing conditions suitable for differentiating and/or expanding lineage specific cells;
thereby generating the lineage-specific cells from the primate embryonic stem cells.